Cellular-resolution imaging is critical in medical diagnosis because it can reveal fine-scale
structures within tissues. Optical coherence tomography (OCT) is a cellular-resolution imaging
technique with the advantages of non-invasive, painless, fast, real-time imaging, etc. The principle
is based on low-coherence interferometry, which yields depth-resolved imaging with micrometerscale
resolution. In clinical practice, the safety, image quality, scanning speed, and tissue
penetration depth of OCT are all important characteristics. Skin cancer is one of the most common
cancers and is among the costliest of all cancers to treat. In our study, we used a Mirau-based fullfield
optical coherence tomography to measure five in vitro skin cells: keratinocyte (HaCaT cell
line), melanocyte, squamous cell carcinoma cell line (A431), and two melanoma cell lines, i.e.,
A375 and A2058. The light source of the Mirau-based FF-OCT system is a cerium-doped yttrium
aluminum garnet (Ce:YAG) single-clad crystal fiber, and generates a 560-nm central wavelength
with a 99-nm full width at half maximum (FWHM). We adopted CNN-based supervised deeplearning
classifiers to discriminate the skin cells and achieved a 95% classification accuracy. This
demonstrates that CNN deep-learning is a powerful tool for identifying cellular-resolution OCT
images. However, obtaining a large number of labeled samples is time-consuming and laborintensive.
In order to reduce label samples in practical applications, we tried to use data
augmentation, self-supervised learning, and transfer learning. These analyses showed interesting
results and efficiently achieved such a goal.