Biosensors Conference

Abstract
We present an integrated smartphone/resistive nanobiosensor for simple, rapid, reagentless, and sensitive detection of OP pesticides in food and environmental water. The biosensor leverages the hydrolytic activity of acetylcholinesterase (AChE) to its substrate, acetylcholine (ACh), and unique transport properties of polyaniline nanofibers (PAnNFs) of hitosan/AChE/PAnNF/carbon nanotube (CNT) nanocomposite film on a gold interdigitated electrode. A mobile app for the biosensor was developed for receiving and analyzing measurement data and displaying and sharing testing results. Under optimal conditions, the biosensor demonstrated a wide linear range (1 ppt–100 ppb) with a low detection limit (0.304 ppt) and high reproducibility (RSD) for Paraoxon-Methyl (PM), a model analyte. Furthermore, the biosensor was successfully applied for analyzing PM spiked food/water samples with an average recovery rate of 98.3% and provided comparable results with liquid chromatography-mass spectrometry. As such, the mobile nanosensing platform open n new avenue for onsite rapid and sensitive monitoring of OP pesticides in food and environmental water.
Biography
: Jun Wang received his Ph.D. in Analytical Chemistry from Wuhan University, China in 2000 and was subsequently awarded a scholarship from DAAD, Germany (2000-2001). Then, he pursued his postdoc research in the California State University at Los Angeles (LA) and the University of California at LA, respectively (2001- 2005). He jointed Pacific Northwest National Laboratory (PNNL) as a scientist (2005-2011). After that, Dr. Wang moved to Charlotte, NC and founded a start-up company (Nanodiagnostic Technology, LLC) in 2011 and served as the president there (2011-present). Since October 2019, Dr. Wang became a Research Professor at the Department of Bioinformatics and Genomics at the University of North Carolina at Charlotte. (APS).

Abstract
Background: Many infections in healthcare facilities are associated with the microbiological quality of the work environment, generally due to poor sanitation. Aim: In order to evaluate the effectiveness of a decontamination protocol (cleaning + disinfection) applied at the “Université des Montagnes” Teaching Hospital, the present study assessed the variation of bacterial loads on surfaces subsequent to decontamination. Susceptibility of bacteria to disinfectants was also evaluated in the same frame. Methodology: This work was conducted with an adjusted bacterial detection/enumeration and susceptibility test protocols and standard bacterial identification protocols. Sampling on surfaces was performed by wet swabbing before cleaning, between cleaning and disinfection and after disinfection. Results: Major findings revealed the predominance of Staphylococcus on target surfaces (75.5%). High bacterial loads recorded on these surfaces before decontamination became undetectable after cleaning with the detergent “Pax lemon”. The majority of isolates (98%) were susceptible to the disinfectants tested, (Surfanios® 0.25% and sodium hypochlorite 0.12%). Conclusion: Overall, these findings indicated process effectiveness on the subjected bacterial populations and suggest the use of either Surfanios® (0.25%) or sodium hypochlorite (0.12%) for work surfaces hygiene, justifying the use of these products in this department for surface decontamination. Also, cleaning with the detergent “Pax lemon” and disinfection with sodium hypochlorite may be sufficient for the types of surfaces subjected in the present research.

Biography
: Hospital hygiene and the means of monitoring microbial presence in the hospital environment in resource-limited settings are among her research interests. He has a Master’s degree in Medical Biology, specializing in Microbiology obtained in 2020 at the Université des Montagnes, Cameroon. He is also interested in antimicrobial resistance issues in human, animal and environmental contexts

Abstract
Background: Objective: Clinically, there is an urgent need for small-diameter artificial vascular grafts (SDAVGs) meeting the requirements of rapid endothelialization and thrombotic resistance, and poor vascular remodeling is one of the bottlenecks affecting the clinical transformation of SDAVGs. This study aims to design and fabricate a three-layer biomimetic SDAVG,and utilize single cell RNA-sequencing to explore the mechanism of the vascular remodeling after SDAVG implantation. Methods: To ensure the safety of the novel three-layer biomimetic SDAVGs in vivo, we evaluated their biocompatibility and hemocompatibility firstly. Then, a porcine carotid artery replacement model was established to analyze the biological performance of the three-layer biomimetic small-diameter artificial vascular graft, and histopathological analysis of tissue sections of vascular grafts was performed by HE and immunohistochemical staining. Additionally, single cell transcriptome sequencing was used to investigate the cell composition and changes after blood vessel implantation. Results: In all in vitro experiments, the small-diameter artificial blood vessel exhibited excellent biocompatibility and hemocompatibility. Furthermore, in vivo transplantation experiments demonstrated favorable anti-thrombotic properties and minimal intimal hyperplasia. And the small-diameter artificial blood caused no evident inflammatory reaction in tissue in vivo implantation experiments. What’s more, single-cell RNA sequencing revealed that endothelial cells (ECs), vascular smooth cells (SMCs), and myeloid cells were the main cell type in the small-diameter artificial blood, and endothelial-to-mesenchymal transition (EndMT) occurred. RNA velocity showed that ECs subtype transitions from quiescent to proliferating phenotype (Figure 1). Conclusion: The biomimetic small-diameter artificial blood vessel has potential clinical application prospects, and the results of single-cell RNA sequencing provide support for its optimization and clinical translation.

Biography
: Yongchun Cui, Female, Professor, Master’s Supervisor in Fuwai Hospital, State Key Laboratory of Cardiovascular Disease, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 102300, China.

Abstract
Background: Title: Perivenular intracavernosal Mesenchymal stem cell ( MSC ) Isolation and storage from Corpora cavernosum and corpora spongiosum in adult male rabbit and adult dog and production of clonal MSC nanoparticulated delivery for penile condition which caused ED ( Erectile dysfunction ) in subject male rabbit and dog and check for the erection function in these male Dog and Rabbit. AIM: To prove the association of whether clonal MSC produced in GMP grade facility has role in ED treatment has value in regenerative medicine practice in Animals and thereby in humans. Methodology: Taking 20 dogs and Rabbit as control and 20 as subjects ( Dogs/ rabbit) DMED ( Stretozocin induced diabetic ED) Male Dogs and Male Rabbits, they are subjected to clonal delivery of nanoparticulated MSC ( Mesenchymal Stem Cells ) . The ED function can be measured by intra cavernosal pressure measurement, apomorphine test, dynamic infusion caversometry, isometric tension study. The to be Stored MSC can be isolated and expanded in culture medium . After extraction these MSC cells are expanded to estimate the cytokine and biological response modifier functions and see for cytokine levels and regeneration mechanisms. Discussion: The nanoparticulated Intracavernosal delivery of MSC can be used if proven superior and can be a potential treatment modality form DMED dogs and Rabbit Model. Conclusion of the Hypothesis: The perivenular MSC taken from potent male Rabbits and Dogs might be able to change the ED status , once proven histologically the treatment could have a potential impact on the treatment of Human Male ED.
Biography
: Dr Sabin Sathyanandan is a Homi Bhabha National Institute, Tata Memorial Centre, International Atomic Energy Agency, Graduate now working as consultant in transfusion medicine services in Sagar Multispecialty Hospital in rural service in lower middle income country, India-south east asia.