Abstract

Background: Many infections in healthcare facilities are associated with the microbiological quality of the work environment, generally due to poor sanitation. Aim: In order to evaluate the effectiveness of a decontamination protocol (cleaning + disinfection) applied at the “Université des Montagnes” Teaching Hospital, the present study assessed the variation of bacterial loads on surfaces subsequent to decontamination. Susceptibility of bacteria to disinfectants was also evaluated in the same frame. Methodology: This work was conducted with an adjusted bacterial detection/enumeration and susceptibility test protocols and standard bacterial identification protocols. Sampling on surfaces was performed by wet swabbing before cleaning, between cleaning and disinfection and after disinfection. Results: Major findings revealed the predominance of Staphylococcus on target surfaces (75.5%). High bacterial loads recorded on these surfaces before decontamination became undetectable after cleaning with the detergent “Pax lemon”. The majority of isolates (98%) were susceptible to the disinfectants tested, (Surfanios^® 0.25% and sodium hypochlorite 0.12%). Conclusion: Overall, these findings indicated process effectiveness on the subjected bacterial populations and suggest the use of either Surfanios^® (0.25%) or sodium hypochlorite (0.12%) for work surfaces hygiene, justifying the use of these products in this department for surface decontamination. Also, cleaning with the detergent “Pax lemon” and disinfection with sodium hypochlorite may be sufficient for the types of surfaces subjected in the present research.

Keywords: Bacteria; Decontamination; Hospital; Surfaces

Abstract

Objective: Clinically, there is an urgent need for small-diameter artificial vascular grafts (SDAVGs) meeting the requirements of rapid endothelialization and thrombotic resistance, and poor vascular remodeling is one of the bottlenecks affecting the clinical transformation of SDAVGs. This study aims to design and fabricate a three-layer biomimetic SDAVG,and utilize single cell RNA-sequencing to explore the mechanism of the vascular remodeling after SDAVG implantation.

Methods: To ensure the safety of the novel three-layer biomimetic SDAVGs in vivo, we evaluated their biocompatibility and hemocompatibility firstly. Then, a porcine carotid artery replacement model was established to analyze the biological performance of the three-layer biomimetic small-diameter artificial vascular graft, and histopathological analysis of tissue sections of vascular grafts was performed by HE and immunohistochemical staining. Additionally, single cell transcriptome sequencing was used to investigate the cell composition and changes after blood vessel implantation.

Results: In all in vitro experiments, the small-diameter artificial blood vessel exhibited excellent biocompatibility and hemocompatibility. Furthermore, in vivo transplantation experiments demonstrated favorable anti-thrombotic properties and minimal intimal hyperplasia. And the small-diameter artificial blood caused no evident inflammatory reaction in tissue in vivo implantation experiments. What’s more, single-cell RNA sequencing revealed that endothelial cells (ECs), vascular smooth cells (SMCs), and myeloid cells were the main cell type in the small-diameter artificial blood, and endothelial-to-mesenchymal transition (EndMT) occurred. RNA velocity showed that ECs subtype transitions from quiescent to proliferating phenotype (Figure 1).

Conclusion: The biomimetic small-diameter artificial blood vessel has potential clinical application prospects, and the results of single-cell RNA sequencing provide support for its optimization and clinical translation.

Abstract

Perivenular intracavernosal Mesenchymal stem cell ( MSC ) Isolation and storage from Corpora cavernosum and corpora spongiosum in adult male rabbit and adult dog and production of clonal MSC nanoparticulated delivery for penile condition which caused ED ( Erectile dysfunction ) in subject male rabbit and dog and check for the erection function in these male Dog and Rabbit.

AIM : To prove the association of whether clonal MSC produced in GMP grade facility has role in ED treatment has value in regenerative medicine practice in Animals and thereby in humans.

Methodology : Taking 20 dogs and Rabbit as control and 20 as subjects ( Dogs/ rabbit) DMED ( Stretozocin induced diabetic ED) Male Dogs and Male Rabbits, they are subjected to clonal delivery of nanoparticulated MSC ( Mesenchymal Stem Cells ) . The ED function can be measured by intra cavernosal pressure measurement, apomorphine test, dynamic infusion caversometry, isometric tension study. The to be Stored MSC can be isolated and expanded in culture medium . After extraction these MSC cells are expanded to estimate the cytokine and biological response modifier functions and see for cytokine levels and regeneration mechanisms.

Discussion : The nanoparticulated Intracavernosal delivery of MSC can be used if proven superior and can be a potential treatment modality form DMED dogs and Rabbit Model.

Conclusion of the Hypothesis : The perivenular MSC taken from potent male Rabbits and Dogs might be able to change the ED status , once proven histologically the treatment could have a potential impact on the treatment of Human Male ED.

Abstract

Background: This study aimed to evaluate the safety and efficacy of allogeneic adipose-derived mesenchymal stem cells (allo-ASC) for the treatment of lateral epicondylosis (LE). Mesenchymal stem cell therapy presents a promising regenerative treatment for tendinopathy, and this trial sought to compare intra-tendon injections of allo-ASC against a control treatment.

Methods: Ten patients with LE persisting for over six months were recruited and randomly assigned to either the cell group or control group. Injections were performed under sonographic guidance, delivering 0.5 ml of thrombin with 10^6 allo-ASCs (cell group) or normal saline (control group) mixed with 0.5 ml of fibrin into the largest hypoechoic defect in the common extensor tendon. Safety assessments were conducted on day 3, and at 2, 6, 12, 24, and 48 weeks post-injection. Efficacy was measured using pain visual analogue scale (VAS) at rest and during activity, Mayo elbow performance index (MEPI), grip strength, and ultrasonographic evaluations at baseline and at 6, 12, 24 and 48 weeks post-injection. Ultrasonographic findings were rated using a 5-point Likert scale. An intention-to-treat analysis with the last observation carried forward method was employed.

Results: Five patients were assigned to the cell group and five to the control group. One control group patient dropped out before the 48-week follow-up due to aggravated calcific tendinitis of the elbow. No serious adverse events were reported. Both groups showed significant improvement in pain VAS at rest and MEPI from baseline to 48 weeks. The cell group also showed significant improvement in pain VAS during activity and grip strength, while the control group did not exhibit significant changes in these measures. No statistically significant differences were observed between the groups at any follow-up point.

Conclusion: This randomized controlled double-blind trial demonstrated that intra-tendon injection of allo-ASC significantly reduced pain during activity over a one-year follow-up compared to baseline. Both allo-ASC and fibrin glue treatments improved pain at rest and function. However, no significant differences between the treatment groups were detected. Future studies with larger sample sizes and refined criteria are recommended.