A novel broad-spectrum antifungal bacterium, Burkholderia gladioli strain NGJ1 that exhibits mycophagy (an ability to forage over fungi), has been identified from rice seedling. NGJ1 deploys a prophage tail-like protein (Bg_9562) to feed on Rhizoctonia solani, a polyphagous fungal pathogen that causes disease in rice, tomato, and many other agriculturally important crops. Moreover, purified protein exhibits strong antifungal activity and upon heterologous overexpression, it imparts fungal disease tolerance in plants. My research work has established that Bg_9562 is also important for endophytic colonization of NGJ1. The protein is delivered into the host apoplast through type 3 secretion system and gets localized into the plasma membrane of host cell, wherein it is perceived as a MAMP (Microbe-associated molecular pattern) to induce defense response. We observed that N-terminal 18-51 amino acid fragment of Bg_9562 has sequence similarity with bacterial flagellin and it is sufficient to promote tolerance against bacterial wilt of tomato. Further using FAM (5´-carboxy fluorescein) tagged Bg_9562 peptide, we demonstrated that it is initially localized in the plasma membrane and thereafter it taken up by the host cells by clathrin-mediated endocytosis. Notably the Bg_9562 peptide perception causes temporary closure of plant stomata and our data anticipate that it mounts abscisic acid-mediated stomatal immunity that imparts bacterial disease tolerance in plants. We are currently analysing the RNA-seq data to get detail insights on Bg-9562 triggered immunity.