{"id":5199,"date":"2026-02-15T07:37:28","date_gmt":"2026-02-15T07:37:28","guid":{"rendered":"https:\/\/cognitionconferences.com\/bacteriology\/?post_type=speaker&p=5199"},"modified":"2026-02-15T07:37:28","modified_gmt":"2026-02-15T07:37:28","slug":"wayne-thomas-shier","status":"publish","type":"speaker","link":"https:\/\/cognitionconferences.com\/bacteriology\/speaker\/wayne-thomas-shier\/","title":{"rendered":"Wayne Thomas Shier"},"content":{"rendered":"

Abstract<\/h2>\n

The persistent global health challenge posed by the emergent spread of drug-resistant Mycobacterium tuberculosis<\/em> (Mtb) has created an urgent need to identify antitubercular agents with novel mechanisms of action that can be used in combination with established antitubercular drugs. The objective of this research was to assess the antitubercular potential of phytochemical components of Datura innoxia <\/em>Mill. (downy thorn-apple) against Mtb <\/em>using in vitro <\/em>and in silico <\/em>methods. Bioassay-guided fractionation and LC-MS\/MS analysis were used for the identification of secondary metabolites with anti-tuberculosis activity in methanolic leaf extracts of D. innoxia. <\/em>The strongest antitubercular activity was exhibited by trans<\/em>-3-indoleacrylic acid with a minimum inhibitory concentration (MIC) of 3.12 \u03bcg\/mL and minimum bactericidal concentration (MBC) of 12.5 \u03bcg\/mL. Screening structural analogs of trans<\/em>-3-indoleacrylic acid for antitubercular activity identified several analogs (indole-3-propionic acid, indole-3-acetonitrile and 3-(1-naphthyl)acrylic acid) with lower antitubercular activity than trans<\/em>-3-indoleacrylic acid.\u00a0 The study of leaf extracts of D. innoxia<\/em> also identified additional components with substantial antitubercular activity.\u00a0 One active component was o-vanillin, which exhibited an MIC of 12.5 \u03bcg\/mL, and an MBC of 50 \u03bcg\/mL.\u00a0 Another active component was 4-n-pentylaniline, which exhibited a MIC of 25 \u03bcg\/mL, and an MBC of 200 \u03bcg\/mL. These D. innoxia<\/em> components are candidates for additional structure-activity relationship studies aiming to identify substances having increased antitubercular potency while maintaining favorable stability and toxicity profiles.<\/p>\n","protected":false},"featured_media":5020,"template":"","meta":{"_acf_changed":false},"schedule":[5],"speaker-category":[6],"class_list":["post-5199","speaker","type-speaker","status-publish","has-post-thumbnail","hentry","schedule-day-1","speaker-category-speakers"],"acf":[],"yoast_head":"\nWayne Thomas Shier | University of Minnesota | USA<\/title>\n<meta name=\"description\" content=\"WT Shier obtained his PhD from the University of Illinois Champaign-Urbana in 1970.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/cognitionconferences.com\/bacteriology\/speaker\/wayne-thomas-shier\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Wayne Thomas Shier | University of Minnesota | USA\" \/>\n<meta property=\"og:description\" content=\"WT Shier obtained his PhD from the University of Illinois Champaign-Urbana in 1970.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/cognitionconferences.com\/bacteriology\/speaker\/wayne-thomas-shier\/\" \/>\n<meta property=\"og:site_name\" content=\"Cognition Conferences\" \/>\n<meta property=\"og:image\" content=\"https:\/\/cognitionconferences.com\/bacteriology\/wp-content\/uploads\/2026\/01\/WooJung-Jon-26.png\" \/>\n\t<meta property=\"og:image:width\" content=\"500\" \/>\n\t<meta property=\"og:image:height\" content=\"600\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/cognitionconferences.com\/bacteriology\/speaker\/wayne-thomas-shier\/\",\"url\":\"https:\/\/cognitionconferences.com\/bacteriology\/speaker\/wayne-thomas-shier\/\",\"name\":\"Wayne Thomas Shier | University of Minnesota | USA\",\"isPartOf\":{\"@id\":\"https:\/\/cognitionconferences.com\/bacteriology\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/cognitionconferences.com\/bacteriology\/speaker\/wayne-thomas-shier\/#primaryimage\"},\"image\":{\"@id\":\"https:\/\/cognitionconferences.com\/bacteriology\/speaker\/wayne-thomas-shier\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/cognitionconferences.com\/bacteriology\/wp-content\/uploads\/2026\/01\/WooJung-Jon-26.png\",\"datePublished\":\"2026-02-15T07:37:28+00:00\",\"description\":\"WT Shier obtained his PhD from the University of Illinois Champaign-Urbana in 1970.\",\"breadcrumb\":{\"@id\":\"https:\/\/cognitionconferences.com\/bacteriology\/speaker\/wayne-thomas-shier\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/cognitionconferences.com\/bacteriology\/speaker\/wayne-thomas-shier\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/cognitionconferences.com\/bacteriology\/speaker\/wayne-thomas-shier\/#primaryimage\",\"url\":\"https:\/\/cognitionconferences.com\/bacteriology\/wp-content\/uploads\/2026\/01\/WooJung-Jon-26.png\",\"contentUrl\":\"https:\/\/cognitionconferences.com\/bacteriology\/wp-content\/uploads\/2026\/01\/WooJung-Jon-26.png\",\"width\":500,\"height\":600},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/cognitionconferences.com\/bacteriology\/speaker\/wayne-thomas-shier\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/cognitionconferences.com\/bacteriology\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Wayne Thomas Shier\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/cognitionconferences.com\/bacteriology\/#website\",\"url\":\"https:\/\/cognitionconferences.com\/bacteriology\/\",\"name\":\"Cognition Conferences\",\"description\":\"\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/cognitionconferences.com\/bacteriology\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Wayne Thomas Shier | University of Minnesota | USA","description":"WT Shier obtained his PhD from the University of Illinois Champaign-Urbana in 1970.","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/cognitionconferences.com\/bacteriology\/speaker\/wayne-thomas-shier\/","og_locale":"en_US","og_type":"article","og_title":"Wayne Thomas Shier | University of Minnesota | USA","og_description":"WT Shier obtained his PhD from the University of Illinois Champaign-Urbana in 1970.","og_url":"https:\/\/cognitionconferences.com\/bacteriology\/speaker\/wayne-thomas-shier\/","og_site_name":"Cognition Conferences","og_image":[{"width":500,"height":600,"url":"https:\/\/cognitionconferences.com\/bacteriology\/wp-content\/uploads\/2026\/01\/WooJung-Jon-26.png","type":"image\/png"}],"twitter_card":"summary_large_image","twitter_misc":{"Est. reading time":"2 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/cognitionconferences.com\/bacteriology\/speaker\/wayne-thomas-shier\/","url":"https:\/\/cognitionconferences.com\/bacteriology\/speaker\/wayne-thomas-shier\/","name":"Wayne Thomas Shier | University of Minnesota | USA","isPartOf":{"@id":"https:\/\/cognitionconferences.com\/bacteriology\/#website"},"primaryImageOfPage":{"@id":"https:\/\/cognitionconferences.com\/bacteriology\/speaker\/wayne-thomas-shier\/#primaryimage"},"image":{"@id":"https:\/\/cognitionconferences.com\/bacteriology\/speaker\/wayne-thomas-shier\/#primaryimage"},"thumbnailUrl":"https:\/\/cognitionconferences.com\/bacteriology\/wp-content\/uploads\/2026\/01\/WooJung-Jon-26.png","datePublished":"2026-02-15T07:37:28+00:00","description":"WT Shier obtained his PhD from the University of Illinois Champaign-Urbana in 1970.","breadcrumb":{"@id":"https:\/\/cognitionconferences.com\/bacteriology\/speaker\/wayne-thomas-shier\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/cognitionconferences.com\/bacteriology\/speaker\/wayne-thomas-shier\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/cognitionconferences.com\/bacteriology\/speaker\/wayne-thomas-shier\/#primaryimage","url":"https:\/\/cognitionconferences.com\/bacteriology\/wp-content\/uploads\/2026\/01\/WooJung-Jon-26.png","contentUrl":"https:\/\/cognitionconferences.com\/bacteriology\/wp-content\/uploads\/2026\/01\/WooJung-Jon-26.png","width":500,"height":600},{"@type":"BreadcrumbList","@id":"https:\/\/cognitionconferences.com\/bacteriology\/speaker\/wayne-thomas-shier\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/cognitionconferences.com\/bacteriology\/"},{"@type":"ListItem","position":2,"name":"Wayne Thomas Shier"}]},{"@type":"WebSite","@id":"https:\/\/cognitionconferences.com\/bacteriology\/#website","url":"https:\/\/cognitionconferences.com\/bacteriology\/","name":"Cognition Conferences","description":"","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/cognitionconferences.com\/bacteriology\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"}]}},"_links":{"self":[{"href":"https:\/\/cognitionconferences.com\/bacteriology\/wp-json\/wp\/v2\/speaker\/5199","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/cognitionconferences.com\/bacteriology\/wp-json\/wp\/v2\/speaker"}],"about":[{"href":"https:\/\/cognitionconferences.com\/bacteriology\/wp-json\/wp\/v2\/types\/speaker"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/cognitionconferences.com\/bacteriology\/wp-json\/wp\/v2\/media\/5020"}],"wp:attachment":[{"href":"https:\/\/cognitionconferences.com\/bacteriology\/wp-json\/wp\/v2\/media?parent=5199"}],"wp:term":[{"taxonomy":"schedule","embeddable":true,"href":"https:\/\/cognitionconferences.com\/bacteriology\/wp-json\/wp\/v2\/schedule?post=5199"},{"taxonomy":"speaker-category","embeddable":true,"href":"https:\/\/cognitionconferences.com\/bacteriology\/wp-json\/wp\/v2\/speaker-category?post=5199"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}